********Title of the dataset:********
Dataset " Anaerobic sulphide removal by haloalkaline sulphide oxidising bacteria"

********Creators:********
R Linssen
Rikke.linssen@wur.nl


********Contributors:********
T Slinkert
CJN Buisman
JBM Klok
A ter Heijne Annemiek.terheijne@wur.nl

********Related publication:********
Anaerobic sulphide removal by haloalkaline sulphide oxidising https://doi.org/10.1016/j.biortech.2022.128435

********Description:********
Recent discoveries show that sulphide oxidising bacteria (SOB) from modern desulphurisation plants are able to spatially separate sulphide removal and oxygen reduction when exposed to intermittent anaerobic and aerobic environments. Here, SOB act as electron shuttles between electron donor and acceptor. The underlying mechanisms for electron shuttling are of yet unknown. To investigate the anaerobic sulphide removal of SOB, batch experiments were performed. SOB harvested from a desulphurisation plant in Wageningen were harvested and made anaerobic in batch bottles. After dosing sulphide, sulphide was measured for 30 minutes. 
The full methodology is described in the material and methods section of the related publication. For Appendix A the method is described in the supplementary information of the related publication. 
The dataset consists of one excel file with several sheets, containing sulphide measurements and NGS data. 

********This dataset contains the following files:********
Data_UptakePaper.xlsx 
First sheet – Figure 2,3,4: Sulphide concentrations [S2-] (mg/L) in time (min) during anaerobic sulphide uptake of SOB. The first row indicates the SOB concentration in mgN/L, the second row indicates the initial sulphide concentration. 
Second sheet – Appendix A: Sulphide concentration [S2-] (mg/L) after different treatments of glassware or dilution of samples for replicas. 
Third sheet – Appendix C: Sulphide concentration [S2-] (mg/L) in time (min) during anaerobic sulphide uptake of SOB and autoclaved SOB. The second row indicates the SOB concentration in mgN/L, the third row indicates the initial sulphide concentration. 
Forth sheet – Appendix D: NGS data of the SOB used during the experiment.

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This dataset is published under the CC BY (Attribution) license.
This license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator.
