Title of the dataset:
Data underlying the publication: Reactor  microbiome enriches vegetable oil with n-caproate and n-caprylate for potential functionalized feed additive production via extractive lactate-based chain elongation  

Authors:
Carlos A. Contreras-Dávila, Norwin Zuidema, Cees J.N. Buisman and David P.B.T.B. Strik
ORCID: Carlos A. Contreras-Dávila, 0000-0001-8356-6242; David P.B.T.B. Strik, 0000-0003-0591-682X
Affiliation: Environmental Technology, Wageningen University & Research

Contact information:
Dr. D.P.B.T.B. Strik
david.strik@wur.nl
Environmental Technology
Wageningen University & Research
Axis-Z, Bornse Weilanden 9, 6708 WG Wageningen (the Netherlands)

Related publication:
Reactor  microbiome enriches vegetable oil with n-caproate and n-caprylate for potential functionalized feed additive production via extractive lactate-based chain elongation
Biotechnology for Biofuels (2021)
DOI : 10.1186/s13068-021-02084-9

Description:
This dataset contains experimental data from batch and continuous chain elongation fermentation with(out) extraction with sunflower oil and oleyl alcohol.

Keywords:
microbial chain elongation, medium-chain carboxylates, extractive fermentation, feed additive, sunflower oil

List of files in the dataset:
[ Calculations constants.csv ]
	Description: constant values used to assess chain elongation fermentation and carboxylates dissociation equilibrium.
[ Extractive batch-Absolute pressure and gas composition.csv ]
	Description: absolute pressure and gas composition measured in extractive batch fermentation experiments.
[ Extractive batch-Carboxylates aqueous phase.csv ]
	Description: carboxylate concentration over time in the aqueous phase of extractive batch experiments.
[ Extractive batch-Carboxylates solvent.csv ]
	Description: carboxylate concentration over time in the organic phase of extractive batch experiments.
[ Extractive batch-pH.csv ]
	Description: pH values over time for extractive batch experiments.
[ ISO standard-Sunflower oil composition.csv ]
	Description: C4 to C23 carboxylate composition of initial and extractive fermentation sunflower oil as determined with the ISO 15885 standard.
[ R1-4-MCC extraction fluxes.csv ]
	Description: calculated nC6 and nC8 extraction rates and fluxes for the biotic and abiotic continuous experiments with sunflower oil as solvent reactors 1 to 4.
[ R1-Carbon and electron selectivity, carbon conversion.csv ]
	Description: calculated selectivity in electron and carbon basis and carbon conversion efficiency (carbon-based yield) for continuous chain elongation in reactor 1.
[ R1-Electron productivities.csv ]
	Description: calculated productivity values in electron basis for continuous chain elongation in reactor 1.
[ R1-Gas production,carbon dioxide balance and effluent pH.csv ]
	Description: measured pH, gas composition and gas production rates, calculated carbon dioxide balance considering pH and carbonates equilibrium for continuous chain elongation in reactor 1.
[ R1-HRT and HCl addition.csv ]
	Description: Measured hydraulic retention time and hydrochloric acid addition for continuous chain elongation in reactor 1.
[ R1-Influent, effluent and solvent carboxylates concentration.csv ]
	Description: measured carboxylates concentration in the influent, effluent and back-extraction samples from sunflower oil solvent for continuous chain elongation in reactor 1.
[ R1-Monitoring raw data.csv ]
	Description: Raw data from regular reactor monitoring and sampling for continuous chain elongation in reactor 1.
[ R1-Yield or stoichiometry.csv ]
	Description: Continuous fermentation consumption/production stoichiometry expressed as yield of +/- mol compound/mol nC6 produced for continuous chain elongation in reactor 1.
[ R2-Carbon and electron selectivity, carbon conversion.csv ]
	Description: calculated selectivity in electron and carbon basis and carbon conversion efficiency (carbon-based yield) for continuous chain elongation in reactor 2.
[ R2-Electron productivities.csv ]
	Description: calculated productivity values in electron basis for continuous chain elongation in reactor 2.
[ R2-Gas production,carbon dioxide balance and effluent pH.csv ]
	Description: measured pH, gas composition and gas production rates, calculated carbon dioxide balance considering pH and carbonates equilibrium for continuous chain elongation in reactor 2.
[ R2-HRT and HCl addition.csv ]
	Description: Measured hydraulic retention time and hydrochloric acid addition for continuous chain elongation in reactor 2.
[ R2-Influent, effluent and solvent carboxylates concentration.csv ]
	Description: measured carboxylates concentration in the influent, effluent and back-extraction samples from sunflower oil solvent for continuous chain elongation in reactor 2.
[ R2-Monitoring raw data.csv ]
	Description: Raw data from regular reactor monitoring and sampling for continuous chain elongation in reactor 2.
[ R2-Yield or stoichiometry.csv ]
	Description: Continuous fermentation consumption/production stoichiometry expressed as yield of +/- mol compound/mol nC6 produced for continuous chain elongation in reactor 2.
[ R3-HRT and HCl addition.csv ]
	Description: measured hydraulic retention time and hydrochloric acid addition in continuous abiotic reactor 3.
[ R3-Influent, effluent and solvent carboxylates concentration.csv ]
	Description: measured carboxylates concentration in the influent, effluent and back-extraction samples from sunflower oil solvent for continuous abiotic reactor 3.
[ R4-HRT and HCl addition.csv ]
	Description: measured hydraulic retention time and hydrochloric acid addition in continuous abiotic reactor 4.
[ R4-Influent, effluent and solvent carboxylates concentration.csv ]
	Description: measured carboxylates concentration in the influent, effluent and back-extraction samples from sunflower oil solvent for continuous abiotic reactor 4.

Methods and materials:
For experimental set-up, variables, quantification and calculation methods, see paper methods and supporting information

License:
This dataset is published under the CC BY (Attribution) license.
This license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator.
