***Nitrous Oxide as a Major Product of Anoxic Bioanode Ammonium Oxidation: Pathway Insights and Implications***

Authors:
Xiaofang Yan, Zhenqiu Yang, Sanne M. de Smit, Dandan Liu, Cees J.N. Buisman, Annemiek ter Heijne .

Environmental technology, Wageningen University & University,

Contact information: Xiaofang.yan@wur.nl

Environmental Technology, Wageningen University & Research, P.O. Box 17, 6700 AA Wageningen, The Netherlands

***General Introduction***

This dataset contains data collected in the chair group Environmental Technology at Wageningen University & Research, as part of Xiaofang Yan's PhD Thesis (chapter 3，4), The data in this data set
was collected between 2023.10-2024.3

This research project was grant by Paqell B.V and China Scholarship Council (CSC).

It is being made public for Publications in order for other researchers to access the raw data.

***Purpose of the test campaign**
To investigated N2O production during NH4+ oxidation at the bioanode (+0.55V vs Ag/AgCl). Our specific focus was on the extent of N2O production and the pathway leading to its formation. 
Additionally, the diversity of N-related functional bacteria was characterized to elucidate the underlying N conversion reactions and the relevance of nitrifier of N2O production was also examined.

***Describe of the data in this data set***

1. N2O production during anoxic bioanodic ammonium oxidation were assessed in a continues mode.
2. The key intermediates, NH2OH and NO2-, were individually supplied as sole substrates under anoxic condition to evaluate their contribution to N2O production.
3. The microbial community compositions were analyzed in both the bioanodic biofilm and the liquid phase analyte.
4. ATU were added to the continuously operated reactor to assess its impact on N2O production and NH4+ oxidation performance.
R1 R2 Represent 2 duplicated reactors running under identical conditions.
