********Valorization of Biobased Biodegradable Plastics and Organic Waste: Microbial and Thermodynamic Insights into Crotonate Fermentation and Chain Elongation********
Authors: Yong Jin 1, Kasper D. de Leeuw 1, and David P. B. T. B. Strik 1
1	Environmental Technology, Wageningen University & Research, Axis-Z, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands; yong.jin@wur.nl 


Corresponding author: David P. B. T. B. Strik
david.strik@wur.nl

Wageningen University & Research - Environmental Technology
Bornse Weilanden 9
6708 WG Wageningen
the Netherlands


********General Introduction********
This dataset contains data collected during crotonate fermentation experiments at Wageningen University & University, as part of Yong Jin's PhD thesis project (November 2021): 
doi: 
It is being made public to act as supplementary data for publications and the PhD thesis of Yong Jin, and other researchers are being asked to use this data in their work. 

The data in this dataset was collected in the Environmental Technology laboratory of the Wageningen University & Research, between November 2021 and December 2025. 
This research project was made possible by the China Scholarship Council (CSC) grant. 

********Purpose of the test campaign********
These experiments aimed to investigate the feasibility of valorizing biobased biodegradable plastic- and organic waste-derived monomers into carboxylates via open-culture fermentation. 
The data shown in this file can validate the results and thermodynamics from the co-fermentation of crotonate and ethanol/lactate. 


********This dataset contains the following files:********
Dataset for CA to C6.xlsx - 
		the first sheet "Constants": The fundamentals of chemicals used for calculation as substrates or products; 
		the second sheet "Concentrations": the concentration of carboxylates detected during the fermentation processes and calculation of carbon balance; 
		the third sheet "Gas-GC 0506": the gas components and percentages detected by two different GC machines, and also the gas pressures measured during the sampling points; 

		
Thermodynamic calculation.xlsx - 
		the first sheet "Constants": the fundamentals of chemicals used for calculation and formulas for the calculation of dissociated/undissociated carboxylates; 
		the second sheet "CA Fermentation": the thermodynamic analysis of crotonate fermentation into acetate and n-butyrate with the consideration of pH, substrate concentration, and product concentration; 
		the third sheet "LA Fermentation": the thermodynamic analysis of lactate fermentation into acetate/propionate or chain elongation to n-butyrate/n-caproate with the consideration of pH, substrate concentration, and product concentration; 
		the fourth sheet "Ethanol Fermentation": the thermodynamic analysis of ethanol oxidation to acetate and chain elongation to n-butyrate/n-caproate with the consideration of pH, substrate concentration, and product concentration; 
		the fifth sheet "Real Fermentation status": the thermodynamic calculation of the co-fermentation of crotonate, ethanol, lactate, and acetate as the substrates into acetate, propionate, n-butyrate, n-caproate, n-butanol with the consideration of pH, substrate concentration, and product concentration in the broth of sampling points; 


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This dataset is published under the CC BY (Attribution) license.
This license allows users to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator.

