***Full Title: "Real-time monitoring of biofilm thickness allows for determination of acetate limitations in bio-anodes"***

João Pereira (a,b), Siqi Pang (a), Casper Borsje (a), Tom Sleutels (a), Bert Hamelers (a,b), Annemiek ter Heijne (b,*)

a Wetsus, European Centre of Excellence for Sustainable Water Technology, Oostergoweg 9, 8911MA, Leeuwarden, the Netherlands
b Environmental Technology, Wageningen University, Bornse Weilanden 9, P.O. Box 17, 6700 AA, Wageningen, the Netherlands

*Corresponding author at: Environmental Technology, Wageningen University, Bornse Weilanden 9, P.O. Box 17, 6700 AA, Wageningen, the Netherlands.

E-mail address: annemiek.terheijne@wur.nl (Annemiek ter Heijne).

***Description of the content***
The data provided in these files have been generated/collected during the study of the development of electro-active biofilms (EABfs) in real-time. These data are made public to share the raw data with other researchers and to serve as supplementary information.
All the experiments were performed at Wetsus, European Center of Excellence for Sustainable Water Technology. 
This work was supported by the “Resource Recovery” theme of Wetsus and the Dutch Research Council (NWO) [project “Understanding and controlling electron flows in electro-active biofilms” with project number 17516 that is part of the research programme Vidi].

***Description of the data in this data set***
Data have been merged in one excel file that contains all the raw data gathered for each of the tested conditions. All the experiments are numbered (from 1 to 12) and organixed in a matrix for moleding purposes. 
All experiments were performed in duplicate (for example, duplicates of the experiment number 5 are labeled as 5.1 or 5.2) 

In each column of the excel file, IVIUM data and measured parameters to characterize the biofilm growth are included. The parameters in the excel file are listed and described below:

Paremeter: exp_num -> number of the experiment;
Parameter: Time (d) -> time of the ivium scan (in days);
Parameter: Anode potential (mV) -> anode potential (vs Ag/AgCl);
Parameter: Influent concentration (mM) -> Acetate concentration in the influent;
Parameter: Biofilm thickness (µm) -> Thickness of the biofilm measured with OCT;
Parameter: Current density (A/m2) -> Calculated current density (in Amperes per electrode surface area)
Parameter: Anolyte concentration (mM) -> Acetate concentration in the anolyte;
Parameter: q_cum (C) -> calculated cumulative charge (in Coulombs);
Parameter: Btot -> buffer concentration in the influent. 
 
The description of the methodolody, model and calculations can be found in the manuscript. DOI: https://doi.org/10.1016/j.biteb.2022.101028

