Data underlying the publication: A guide to the in vitro reconstitution of Cdc42 GTPase activity and its regulation

Datacite citation style

Tschirpke, Sophie; Laan, Liedewij; van Opstal, Frank; van der Valk, Ramon; Daalman, Werner (2023): Data underlying the publication: A guide to the in vitro reconstitution of Cdc42 GTPase activity and its regulation. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/ddc72065-1082-4183-908d-a55b04d05cb2.v1
Other citation styles (APA, Harvard, MLA, Vancouver, Chicago, IEEE) available at Datacite

Dataset

Version 2 - 2025-06-06 (latest)
Version 1 - 2023-07-17

We investigated the in vitro GTPase activity of the S.cerevisae GTPase Cdc42, alone and in combination with its GDP/GTP exchange factor (GEF) Cdc24. We used the GTPase assay 'GTPase-Glo assay' by Promega. The data consists of assay data (amount of remaining GTP for various time points and protein concentrations) for variously tagged Cdc42 constructs and purification batches.

History

  • 2023-07-17 first online, published, posted

Publisher

4TU.ResearchData

Format

xlsx

Funding

  • funding from the European Research Council under the European Union’s Horizon 2020 research and innovation programme (grant agreement 758132) (grant code 758132) European Research Council
  • funding from the Netherlands Organization for Scientific Research (Nederlandse Organisatie voor Wetenschappelijk Onderzoek) through a Vidi grant (016.Vidi.171.060) (grant code 016.Vidi.171.060) Netherlands Organization for Scientific Research

Organizations

TU Delft, Faculty of Applied Sciences, Department of Bionanoscience
TU Delft, Kavli Institute of Nanoscience

DATA

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