Data underlying the publication "Equibiaxial and uniaxial cyclic strain similarly affect Notch signaling and vascular smooth muscle cell phenotype in 2D"
DOI: 10.4121/6964ba10-be3a-4673-ba90-fb554ec751dd
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Dataset
Licence CC BY 4.0
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This study explored how strain anisotropy affects mechanosensitive Notch signaling and the resulting phenotype of vascular smooth muscle cells (VSMCs). We applied cyclic strains within the physiological range to compare the effects of equibiaxial and uniaxial loading on Notch activity and phenotypic changes in both synthetic and contractile VSMCs. By assessing three deformation parameters, we aimed to identify the main strain feature influencing these responses. Our findings indicate that VSMCs react similarly to both strain types, suggesting that strain anisotropy may not have a significant impact on Notch signaling and phenotype modulation.
This dataset contains the videos and data for strain analysis, immunofluorescence staining images, and real-time polymerase chain reaction data.
History
- 2025-05-20 first online, published, posted
Publisher
4TU.ResearchDataFormat
strain analysis videos (.avi), strain analysis data (.csv), qPCR data (.csv), fluorescence microscopy images (.tif)Associated peer-reviewed publication
Equibiaxial and uniaxial cyclic strain similarly affect Notch signaling and vascular smooth muscle cell phenotype in 2DFunding
- Predicting cardiovascular regeneration: integrating mechanical cues and signaling pathways (grant code 802967) [more info...] European Research Council
- The integration of cell signalling and mechanical forces in vascular morphology (grant code 771168) [more info...] European Research Council
Organizations
TU Eindhoven, Department of Biomedical Engineering, Eindhoven, The NetherlandsTU Eindhoven, Institute for Complex Molecular Systems, Eindhoven, The Netherlands
Åbo Akademi University, Faculty of Science and Engineering, Turku, Finland
DATA
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- 231,625,716 bytesMD5:
fd8e09fbe6e6f9d210007a9b50c31d4b
data_Karakaya_IntegrativeBiology.zip