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DOI: 10.4121/529c9e42-6261-4591-ac87-3939a3686bd7

Beije, Jaïrus; Bokinsky, Gregory (2025): Data underlying Chapter 2 of PhD thesis: Filament mediated negative feedback regulation of phospholipid synthesis sufficient for coupling growth and membrane synthesis in Escherichia coli. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/529c9e42-6261-4591-ac87-3939a3686bd7.v1

Categories

• Microbiology
• Biochemistry and Cell Biology
• Biological Sciences

Keywords

E. coli fluorescence microscopy PslB

Licence

CC BY-SA 4.0

Interoperability

• RO-Crate Metadata

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by Jaïrus Beije , Gregory Bokinsky

This collection of microscopy images, datasets, and analysis scripts supports an investigation of PlsB regulation and localization in Escherichia coli. The goal of this research is to establish if filamentation of PlsB mediated by membrane abundance is the regulatory mechanism by which the E. coli cell regulates phospholipid synthesis. The growth rate dataset establishes that PlsB-msGFP2 expression does not significantly influence cellular physiology under varied carbon sources. Multiple microscopy datasets capture static and time-lapse fluorescence imaging of E. coli under diverse metabolic, antibiotic, and mutant conditions, revealing how PlsB localization and clustering respond to perturbations. The accompanying MATLAB pipelines enable reproducible quantification of foci number, spatial distribution, and cell morphology from both single timepoint and timelapse experiments. Complementary proteomics measurements quantify PlsB peptide abundance in wild-type and msGFP2-tagged strains. Together, these datasets and analytical tools form an integrated framework for studying the coupling between growth, lipid synthesis, and membrane-associated protein organization in E. coli.

History

• 2025-10-23 first online, published, posted

Publisher

4TU.ResearchData

Format

Image(tif, ND2), spreadsheet(excel), LCMS data (.sky), miscellaneous(.mat, .xml)

Organizations

TU Delft, Faculty of Applied Sciences, Kavli Institute of Nanoscience, Bionanoscience Department, Bokinsky Lab