Single-molecule microscopy data related to "Telomeres stall DNA loop extrusion by condensin"

DOI:10.4121/4dae22e3-0eed-4f9b-8c47-3d4555010e3e.v1
The DOI displayed above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
DOI: 10.4121/4dae22e3-0eed-4f9b-8c47-3d4555010e3e

Datacite citation style

Analikwu, Brian; Deshayes, Alice; van der Torre, Jaco; Guérin, Thomas; Katan, Allard et. al. (2025): Single-molecule microscopy data related to "Telomeres stall DNA loop extrusion by condensin". Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/4dae22e3-0eed-4f9b-8c47-3d4555010e3e.v1
Other citation styles (APA, Harvard, MLA, Vancouver, Chicago, IEEE) available at Datacite

Dataset

Single-molecule imaging data files of DNA loop extrusion by yeast condensin on DNA with Rap1 protein arrays. Both whole fields of view (FOVs) and single cropped DNA molecules are deposited.


The dataset is part of a cell biology and biophysics study of how the process of DNA loop extrusion by condensin interacts with multiple roadblocks on DNA. We found that arrays of proteins may stall DNA loop extrusion dependent on their length and density, both in vivo and in vitro.


The data was generated using single-molecule HILO fluorescence microscopy on DNA (intercalating fluorophores) and fluorescently labeled proteins (Rap1 and LacI proteins). This subset of data gives a representative overview of the data that was obtained for the related journal article. It serves as a demonstration of the type of data and the type of events that were analyzed.


The README file contains more specific information on the imaging and file structure/naming.

History

  • 2025-06-20 first online, published, posted

Publisher

4TU.ResearchData

Format

.tiff

Associated peer-reviewed publication

Telomeres stall DNA loop extrusion by condensin

Funding

  • BaSyC program of NWO-OCW
  • ERC Advanced Grant 883684 (grant code 883684) ERC
  • NWO grant OCENW.GROOT.2019.012 (grant code OCENW.GROOT.2019.012) NWO
  • ANR-15CE12-0007 DNA-Life
  • ANR-14-CE10-021 DICENS

Organizations

Kavli Institute of Nanoscience, Delft University of Technology;
Université Paris-Saclay, Université Paris-Cité, CEA, Inserm, Institut de biologie François Jacob;
Institut Curie

DATA

Files (5)